Plant material
The mapping population used in this study (A05141) consisted of 162 F1 progeny derived from a cross between ‘Rio Grande Russet’ (female parent) and ‘Premier Russet’ (male parent). ‘Premier Russet’ is a dual purpose (processing and fresh market) potato variety with significant resistance to the accumulation of reducing sugars after long-term storage at low temperatures . Rio Grande Russet is a high yielding fresh market potato variety . A05141 was developed at the USDA-ARS Small Grains escort Centennial and Potato Germplasm Research Unit (Aberdeen, ID) and was one of the mapping populations phenotyped and genotyped by the Solanaceae Coordinated Agricultural Project (SolCAP) .
Phenotypic investigation
Job studies was presented from the USDA-ARS, School regarding Idaho Research and you will Expansion Cardio (Aberdeen, ID) and you can Vermont County University Tidewater Lookup Station (Plymouth, NC) inside summers from 2010 and you will 2011. New progeny and you will parents was indeed analyzed inside the a beneficial randomized over cut-off design with a few replications from ten-slope plots at each and every location. Vine maturity is actually aesthetically scored on the a-1–9 measure from the 95 (95DAP) and 120 (120DAP) weeks after planting, with step one = very early, 100% vines inside the a story dead, when you find yourself nine = very later, complete flower which have buds obvious. Plant progress behavior try submitted early in blooming and shown while the step three = upright (> 45° with floor), 5 = semi-upright (30–45° having floor), and eight = dispersed (More document ten: Table S5). Verticillium wilt and you can very early blight (EB) (Altenaria tenuis) diseases have been ranked into the a 0–nine level. For EB, 0 indicates zero disease and you will nine > 90% of your own leaf affected area in a storyline. To own VW, the size and style is a blended get to possess leaf town/base flagging as a result of the disease (Most file 10: Table S5). Plots of land were not inoculated, but Aberdeen areas keeps highest inoculum of Verticillium throughout the surface, collected more than decades. Very early blight is observed annual at the ID and you can NC towns as well as for that it trial the condition is allowed to create without a doubt, no fungicides removed their handle.
Stores criteria
Tuber examples was basically addressed with Isopropyl Chlorpropham (N-(3-chlorophenyl) carbamate) at a rate from twenty two ppm to quit sprouting and you may kept on 42 °F and you can 95% relative humidity for ninety days.
Sugar determinations
Sugar levels toward solutions inside trial was determined away from a great 10-tuber decide to try with the method of Sowokinos et al. with modifications. Tubers were cut using an enthusiastic Kut Shoe Stringer French fry cutter. 2 hundred grams regarding tuber tissues built-up throughout the center away from the newest 10 tubers had been macerated inside a keen Acme Juicerator (Acme Equipment, Spring Slope, FL). Through the control, tuber cells is actually sparkling having 150 mL of sodium-phosphate buffer (0.05 Meters, pH eight.5) to possess a last homogenate number of 275 mL. Sugar concentrations were computed playing with an effective YSI design 2700 Analyzer (Red Springs Appliance Co., Inc., Red-colored Springs, OH) and you can shown to the a % new lbs base.
Bud-end and you may Base-avoid fry color
A remove off tissues (3.0 cm ? 0.8 cm) taken off each of the ten tubers included in the brand new sugar removal process was utilized getting fry colour dedication (10 strips for each and every simulate). Pieces was in fact fried from inside the canola oils at 191 °C getting step three.5 min. Fry colour was determined within this 3 minute having fun with a product 577 Photovolt Meditation Meter (model 577, Photovolt Products Inc., Minneapolis, MN). An eco-friendly filter out was utilized and the appliance was calibrated having fun with a black colored-hole important since 0.0% reflectance and you can a light plaque (Cat. Zero. 26-5Cat. Zero. 26–570-08) because 99.9% reflectance. Specifications was indeed taken on the newest stops of each remove. The lower the fresh new reflectance measurement, new black new fry color and you may the other way around.