step three.step 1 Phenotype
We amassed phenotypic data on Vida/MTHW0202 RIL people and you will moms and dads xxx inside about three rainfed as well as 2 irrigated surroundings inside the 2013?2014 expanding season. Desk 1 records setting labeled toward combined, rainfed, and you will irrigated environments for www.datingranking.net/local-hookup/vancouver-2 a few characteristics which were built-up on moms and dads and RIL society. The rainfed grains yield RIL mean is actually thirty five% below the newest irrigated RIL grain yield imply. At the same time, rainfed produce part attributes decide to try weight, thousand-kernel weight, and you can seed matter for every single head were cuatro, eight, and you may seven% below this new irrigated surroundings, correspondingly. Alternatively, effective tiller number produce parts RIL suggest is 8% highest regarding the rainfed surroundings than the irrigated environment.
- Mention. GLDAH, green leaf stage after heading, a description of one’s stay-green trait.
- * Significant difference one of RIL mothers and RIL records in the .05 possibilities level.
- ** Significant difference certainly RIL mothers and you may RIL records within .01 probability top.
- *** Factor one of RIL parents and you will RIL records during the .001 possibilities height.
Grain yield among parents was significantly different (P < .05) in only the rain-fed environments where Vida's grain yield was 6.0% higher than MTHW0202. MTHW0202 had significantly higher (P < .05) seed diameter and seed number per head across, rainfed, irrigated, and combined environments. Vida had significantly higher productive tiller number (P < .01) vs. MTHW0202 in all environments. Vida also had significantly later heading date (P < .001) and maturity date (P < .05) vs. MTHW020 across combined, rainfed, and irrigated environments, but GLDAH was not significantly different among parents in any environment. Thousand-kernel weight was significantly higher (P < .01) in MTHW0202 vs. Vida in the combined and irrigated environments. Vida was significantly taller (P < .05) than MTHW0202 in the combined and rainfed environments.
Significant differences among RIL entry means were also observedbined environment RIL entry means were significantly different for test weight (P < .001), thousand-kernel weight (P < .001), seed diameter (P < .001), seed number per head (P < .001), heading date (P < .001), GLDAH (P < .05), maturity (P < .05), plant height (P < .001), and grain protein content (P < .001) (Table 1). Significant differences among RIL entry means were also observed for thousand-kernel weight (P < .001), seed number per head (P < .001), plant height (P < .001), and grain protein content (P < .001) in rainfed environments. Plant height RIL entry means were significantly different (P < .001) in the irrigated environments. Transgressive segregation was observed in the RIL population for all traits in the combined, rainfed, and irrigated environments, except for GLDAH in the irrigated environments and maturity in the rainfed environments.
step 3.dos Relationship analysis
Pearson correlations between traits varied among environments. In the rainfed environments, GLDAH was positively correlated with yield (P < .001, r = .37), test weight (P < .001, r = .47), and thousand-kernel weight (P < .001, r = .38), but was negatively correlated with seed number per head (P < .05, r = ?.20) and heading date (P < .001, r = ?.36) (Table 2). In irrigated environments, GLDAH was positively correlated with test weight (P < .01, r = .25) and thousand-kernel weight (P < .01, r = .24), but was not significantly correlated with yield (Table 2). Seed number per head (P < .05, r = ?.20) and heading date (P < .001, r = ?.39) were negatively correlated with GLDAH in irrigated environments (Table 2). Yield was positively correlated with test weight (P < .001, r = .36) and thousand-kernel weight (P < .01, r = .24), but was negatively correlated with heading date (P < .001, r = ?.31) in rainfed environments (Table 3). Yield was positively correlated with seed number per head (P < .001, r = .33), heading date (P < .05, r = .19), and productive tiller number (P < .001, r = .29), but was not significantly correlated with test weight and thousand-kernel weight in irrigated environments (Table 3).